Vincent Mulholland

Molecular Biology Unit Manager

Telephone Number
+44(0)131 244 8845

Email Address
vince.mulholland@sasa.gsi.gov.uk

Research interests/Areas of expertise

Application of molecular biology methods to the detection and characterisation of organisms of agricultural/horticultural interest.
Use of molecular methods in detection and quantification of GMO contamination of seed.

Biography

Joined SASA in 1995, and worked on a variety of molecular biology projects. This included potato cyst nematode identification, potato virus detection and characterization, detection and quantification of fungal pathogens of wheat, GMO detection, plant pathogen genomics, etc.
For the 10 years prior to 1995 I worked on bacterial genetics research projects at Newcastle, Edinburgh and Warwick Universities. This included five years studying the molecular genetic systems of the plant pathogen Pectobacterium atrosepticum.

Publications

Palmano S, Mulholland V, Kenyon DM, Saddler GS, Jeffries CJ. 2015. Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes. Plant Pathology : Techniques and Protocols. :113-122.
Mulholland V, Elliot M, Green S. 2015. Diagnostics of Tree Diseases Caused by Phytophthora Species. Plant Pathology : Techniques and Protocols. :59-74.
Reid A, Evans FF, Mulholland V, Cole Y, Pickup J. 2015. High-Throughput Diagnosis of Potato Cyst Nematodes in Soil Samples. Plant Pathology : Techniques and Protocols. :137-148.
Mulholland V, Schlenzig A, MacAskill GA, Green S. 2013. Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain. Forest Pathology. 43(6):513–517.
Mulholland V, MacAskill GA, Laue BE, Steele H, Kenyon DM, Green S. 2012. Development and verification of a diagnostic assay based on EF-1 α for the identification of Armillaria species in Northern Europe. Forest Pathology. 42(3):229-238.
A'Hara D, Mulholland V, Ventisei HM. 2012. Development of a multiplex real-time quantitative PCR assay to detect phoma pathogens of potato.
A'Hara D, Mulholland V, Ventisei HM. 2012. Development of a multiplex real-time quantitative PCR assay to detect Phoma pathogens of potato. Proceedings Crop Protection in Northern Britain 2012. :279-284.
Kelly RM, Cahill G, Elphinstone JG, Mitchell WJ, Mulholland V, Parkinson NM, Pritchard L, Toth IK, Saddler GS. 2012. Development of a real-time PCR assay for the detection of ‘Dickeya solani’. Proceedings Crop Protection in Northern Britain 2012. :201-206.
Schlenzig A, Campbell R, Mulholland V. 2011. Thuja occidentalis: a new host for Phytophthora lateralis. New Disease Reports. 24:8.
Mortensen RJ, Shen X, Reid A, Mulholland V. 2010. Characterisation of viruses infecting potato plants from a single location in Shetland, an isolated Scottish archipelago. Journal of Phytopathology. 158(9):633-640.
Kenyon DM, Reid A, Evans FF, Mulholland V, Pickup J, Blok VC, Paterson A, Phillips MS. 2010. Development of a high-throughput method for the detection and speciation of PCN. Proceedings Crop Protection in Northern Britain 2010. :201-206.
Reid A, Kenyon DM, Evans FF, Mulholland V, Pickup J, Blok VC, Paterson A, Phillips MS. 2010. Development of a high-throughput method for the detection and species determination of PCN.. Aspects of Applied Biology. 103, 3rd Symposium on Potato Cyst Nematodes, Harper Adams University College, Newport, UK:11-14.
Palmano S, Jeffries CJ, Mulholland V, Saddler GS. 2010. LNA probe-based Real-Time PCR for the detection of phytoplasmas in Solanum tuberosum. COST Action FA0807 Conference. Current status and perspectives of phytoplasma disease research and management.. :AbstractBook,p30.
Mulholland V, Mortensen RJ, Shen X, Reid A. 2010. Potato viruses: a snapshot from Shetland. SGM Conference, March 2010.
Sharp EA, Giela A, Mulholland V, Taylor MJ. 2009. Combining Analytical Chemistry and DNA Barcoding techniques to identify chemicals and species involved in the poisoning of vertebrate wildlife in Scotland. chem poster - Combining analytical chemistry and DNA barcoding.pdf (1.3 MB)
Mulholland V. 2009. Immunocapture-PCR for plant virus detection. Plant Pathology : Techniques and Protocols. 508:183-192.
James T, Mulholland V, Jeffries CJ, Chard J. 2008. First report of Tomato chlorotic dwarf viroid infecting commercial petunia stocks in the United Kingdom. Plant Pathology. 57(2):400.
Mulholland V, Sharp EA, Giela A, Taylor MJ. 2008. Identification of animal tissue in support of WIIS. Identification of animal tissue in support of WIIS.pdf (1.69 MB)
James T, Mulholland V, Jeffries CJ, Chard J. 2007. First report of Tomato chlorotic dwarf viroid infecting commercial Petunia stocks in the United Kingdom. New Disease Reports. 15:42.
Nisbet C, Butzonitch I, Colavita M, Daniels J, Martin J, Burns R, George E, Akhond MAY, Mulholland V, Jeffries CJ. 2006. Characterization of potato rough dwarf virus and potato virus P: distinct strains of the same viral species in the genus Carlavirus. Plant Pathology. 55(6):803-812.
Gabriel DW, Allen C, Schell M, Denny TP, Greenberg JT, Duan YP, Flores-Cruz Z, Huang Q, Clifford JM, Presting G et al.. 2006. Identification of Open Reading Frames Unique to a Select Agent: Ralstonia solanacearum Race 3 Biovar 2. Molecular Plant-Microbe Interactions. 19(1):69-79.
Thomas JE, Kenyon DM, Law JR, Bates J, Cockerell V, Mulholland V. 2005. Development and application of a quantitative PCR test for detection of Microdochium nivale on wheat seed. BCPC International Congress – Crop Science & Technology 2005. :201-205.
Mulholland V. 2005. Immunocapture-Polymerase Chain Reaction. Immunochemical Protocols . :281-290.
Cockerell V, Paveley ND, Clark WS, Thomas JE, Anthony S, McEwan M, Roberts AMI, Bates J, Law J, Kenyon DM et al.. 2004. Cereal seed health and seed treatment strategies: Exploiting new seed testing technology to optimise seed health decisions for wheat.
Mulholland V, Davey JC, Kerr J, Saddler GS. 2004. Molecular methods in support of the Scottish Executive's GM Inspectorate. Proceedings Crop Protection in Northern Britain 2004. :57-62.
McNeil M, Roberts AMI, Cockerell V, Mulholland V. 2004. Real-time PCR assay for quantification of Tilletia caries contamination of UK wheat seed. Plant Pathology. 53(6):741-750.
Browning I, Fox A, Holmes R, Wilson D, Mulholland V, Darling D. 2004. Variation in Potato Virus Y strains in Scottish seed potatoes. Proceedings Crop Protection in Northern Britain 2004. :327-331.
Cockerell V, Mulholland V, McEwan M, Paveley ND, Clark WS, Anthony S, Thomas JE, Bates J, Kenyon DM. 2002. Control of seed and soil-borne diseases: importance of seed health. HGCA R&D Conference 2002, Agronomic intelligence: the basis for profitable production. :3.1-3.11.
McEwan M, Mulholland V. 2002. Development of molecular diagnostic assays for seed-borne pathogens of wheat. Proceedings Crop Protection in Northern Britain 2002. :127-132. Development of molecular diagnostic assays for seed-borne pathogens of wheat.pdf (99.53 KB)
Brattey C, Badge JL, Burns R, Foster GD, George E, Goodfellow HA, Mulholland V, McDonald JG, Jeffries CJ. 2002. Potato latent virus: a proposed new species in the genus Carlavirus. Plant Pathology. 51(4):495–505.
Cockerell V, Mulholland V, McEwan M, Paveley ND, Clark WS, Anthony S, Thomas JE, Bates J, Kenyon DM, Taylor EJA. 2001. Seed treatment according to need in winter wheat. BCPC Symposium Proceedings No. 76: Seed Treatment: Challenges and Opportunities. :111-116.
Mulholland V, McEwan M. 2000. PCR-based diagnostics of Microdochium nivale and Tilletia caries infecting winter wheat seed. EPPO Bulletin. 30(3-4):543-547.
Toth IK, Thorpe CJ, Bentley S, Mulholland V, Hyman LJ, Perombelon MCM, Salmond GPC. 1999. Mutation in a gene required for lipopolysaccharide and enterobacterial common antigen biosynthesis affects virulence in the plant pathogen Erwinia carotovora subsp. atroseptica. Molecular PlantMicrobe Interactions. 12:499-507.
Mulholland V, McEwan M, Cockerell V. 1999. Quantification of Microdochium nivale and Tilletia caries on winter wheat seed, using the PCR MIMIC technique. Proceedings of the 3rd International Seed Testing Association Plant Disease Committee, Seed Health Symposium . :86-90.
Mulholland V. 1998. PCR detection and quantification of a fungal pathogen of cereals. Abstracts of the 7th International Congress of Plant Pathology.
Toth IK, Mulholland V, Cooper V, Bentley S, Shih Y-L, Perombelon MCM, Salmond GPC. 1997. Generalised transduction in the blackleg pathogen Erwinia carotovora subspecies atroseptica by bacteriophage. Microbiology. 143(7):2433-2438.
Mulholland V, Carde L, O'Donnell K, Fleming CC, Powers TO. 1996. Use of the polymerase chain reaction to discriminate potato cyst nematode at the species level. BCPC Symposium Proceedings No. 65: Diagnostics in Crop Production. :247-252.